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proteoextract subcellular proteome extraction kit  (Millipore)


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    Structured Review

    Millipore proteoextract subcellular proteome extraction kit
    Proteoextract Subcellular Proteome Extraction Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/proteoextract%C2%AE+subcellular+proteome+extraction+kit/proteoextract+subcellular+proteome+extraction+kit/us12365898-448-96-101
    Average 90 stars, based on 1 article reviews
    proteoextract subcellular proteome extraction kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Fractionation:

    Article Title: Two opposing gene expression patterns within ATRX aberrant neuroblastoma
    Article Snippet: Cell fractionation experiments were performed with the ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem®).

    Article Title: Helicobacter pylori regulates TIFA turnover in gastric epithelial cells.
    Article Snippet: When appropriate, protein amount was estimated using Pierce BCA protein assay kit (Thermo Fisher Scientific).

    Article Title: Piezo1 ion channels are capable of conformational signaling
    Article Snippet: The membrane fraction was isolated from Neuro2A cells using the ProteoExtract® Subcellular Proteome Extraction Kit (Sigma) and concentrated to 1-2 mg/mL using a Pierce TM concentrator (3K molecular weight cutoff; ThermoFisher Scientific).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: ProteoExtract® Subcellular Proteome Extraction Kit , Millipore-Sigma , 539790.

    Article Title: Pre-clinical characterization of novel multi-client inhibitors of Sec61 with broad anti-tumor activity
    Article Snippet: 243 Proteomic profiling 244 Cell lines and PBMCs were treated for 24 hours with either KZR-834 or KZR-261 245 (250 nM) followed by subcellular fractionation of cytosolic (F1) and 246 membrane/organelle (F2) proteomes using the ProteoExtract® Subcellular 247 Proteome Extraction Kit (Calbiochem).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: Cytoplasm and membrane fractions were obtained using the ProteoExtract® Subcellular Proteome Extraction Kit (Millipore-Sigma] following manufacturer protocol.

    Article Title: Linoleic acid improves PIEZO2 dysfunction in a mouse model of Angelman Syndrome.
    Article Snippet: Transfected, Latrunculin A (1μM; 20–24 h)-treated, or LAsupplemented MCC13 cells were treated with ProteoExtract® Subcellular Proteome Extraction Kit (Millipore Sigma) to obtain cytosolic, membrane, and cytoskeletal fractions, according to the manufacturer’s instructions and elsewhere100.

    Membrane:

    Article Title: Two opposing gene expression patterns within ATRX aberrant neuroblastoma
    Article Snippet: Cell fractionation experiments were performed with the ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem®).

    Article Title: Helicobacter pylori regulates TIFA turnover in gastric epithelial cells.
    Article Snippet: When appropriate, protein amount was estimated using Pierce BCA protein assay kit (Thermo Fisher Scientific).

    Article Title: Piezo1 ion channels are capable of conformational signaling
    Article Snippet: The membrane fraction was isolated from Neuro2A cells using the ProteoExtract® Subcellular Proteome Extraction Kit (Sigma) and concentrated to 1-2 mg/mL using a Pierce TM concentrator (3K molecular weight cutoff; ThermoFisher Scientific).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: ProteoExtract® Subcellular Proteome Extraction Kit , Millipore-Sigma , 539790.

    Article Title: Pre-clinical characterization of novel multi-client inhibitors of Sec61 with broad anti-tumor activity
    Article Snippet: 243 Proteomic profiling 244 Cell lines and PBMCs were treated for 24 hours with either KZR-834 or KZR-261 245 (250 nM) followed by subcellular fractionation of cytosolic (F1) and 246 membrane/organelle (F2) proteomes using the ProteoExtract® Subcellular 247 Proteome Extraction Kit (Calbiochem).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: Cytoplasm and membrane fractions were obtained using the ProteoExtract® Subcellular Proteome Extraction Kit (Millipore-Sigma] following manufacturer protocol.

    Article Title: Linoleic acid improves PIEZO2 dysfunction in a mouse model of Angelman Syndrome.
    Article Snippet: Transfected, Latrunculin A (1μM; 20–24 h)-treated, or LAsupplemented MCC13 cells were treated with ProteoExtract® Subcellular Proteome Extraction Kit (Millipore Sigma) to obtain cytosolic, membrane, and cytoskeletal fractions, according to the manufacturer’s instructions and elsewhere100.

    Extraction:

    Article Title: Two opposing gene expression patterns within ATRX aberrant neuroblastoma
    Article Snippet: Cell fractionation experiments were performed with the ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem®).

    Article Title: Helicobacter pylori regulates TIFA turnover in gastric epithelial cells.
    Article Snippet: When appropriate, protein amount was estimated using Pierce BCA protein assay kit (Thermo Fisher Scientific).

    Article Title: Piezo1 ion channels are capable of conformational signaling
    Article Snippet: The membrane fraction was isolated from Neuro2A cells using the ProteoExtract® Subcellular Proteome Extraction Kit (Sigma) and concentrated to 1-2 mg/mL using a Pierce TM concentrator (3K molecular weight cutoff; ThermoFisher Scientific).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: ProteoExtract® Subcellular Proteome Extraction Kit , Millipore-Sigma , 539790.

    Article Title: Pre-clinical characterization of novel multi-client inhibitors of Sec61 with broad anti-tumor activity
    Article Snippet: 243 Proteomic profiling 244 Cell lines and PBMCs were treated for 24 hours with either KZR-834 or KZR-261 245 (250 nM) followed by subcellular fractionation of cytosolic (F1) and 246 membrane/organelle (F2) proteomes using the ProteoExtract® Subcellular 247 Proteome Extraction Kit (Calbiochem).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: Cytoplasm and membrane fractions were obtained using the ProteoExtract® Subcellular Proteome Extraction Kit (Millipore-Sigma] following manufacturer protocol.

    Article Title: Linoleic acid improves PIEZO2 dysfunction in a mouse model of Angelman Syndrome.
    Article Snippet: Transfected, Latrunculin A (1μM; 20–24 h)-treated, or LAsupplemented MCC13 cells were treated with ProteoExtract® Subcellular Proteome Extraction Kit (Millipore Sigma) to obtain cytosolic, membrane, and cytoskeletal fractions, according to the manufacturer’s instructions and elsewhere100.

    Isolation:

    Article Title: Two opposing gene expression patterns within ATRX aberrant neuroblastoma
    Article Snippet: Cell fractionation experiments were performed with the ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem®).

    Article Title: Helicobacter pylori regulates TIFA turnover in gastric epithelial cells.
    Article Snippet: When appropriate, protein amount was estimated using Pierce BCA protein assay kit (Thermo Fisher Scientific).

    Article Title: Piezo1 ion channels are capable of conformational signaling
    Article Snippet: The membrane fraction was isolated from Neuro2A cells using the ProteoExtract® Subcellular Proteome Extraction Kit (Sigma) and concentrated to 1-2 mg/mL using a Pierce TM concentrator (3K molecular weight cutoff; ThermoFisher Scientific).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: ProteoExtract® Subcellular Proteome Extraction Kit , Millipore-Sigma , 539790.

    Article Title: Pre-clinical characterization of novel multi-client inhibitors of Sec61 with broad anti-tumor activity
    Article Snippet: 243 Proteomic profiling 244 Cell lines and PBMCs were treated for 24 hours with either KZR-834 or KZR-261 245 (250 nM) followed by subcellular fractionation of cytosolic (F1) and 246 membrane/organelle (F2) proteomes using the ProteoExtract® Subcellular 247 Proteome Extraction Kit (Calbiochem).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: Cytoplasm and membrane fractions were obtained using the ProteoExtract® Subcellular Proteome Extraction Kit (Millipore-Sigma] following manufacturer protocol.

    Article Title: Linoleic acid improves PIEZO2 dysfunction in a mouse model of Angelman Syndrome.
    Article Snippet: Transfected, Latrunculin A (1μM; 20–24 h)-treated, or LAsupplemented MCC13 cells were treated with ProteoExtract® Subcellular Proteome Extraction Kit (Millipore Sigma) to obtain cytosolic, membrane, and cytoskeletal fractions, according to the manufacturer’s instructions and elsewhere100.

    Molecular Weight:

    Article Title: Two opposing gene expression patterns within ATRX aberrant neuroblastoma
    Article Snippet: Cell fractionation experiments were performed with the ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem®).

    Article Title: Helicobacter pylori regulates TIFA turnover in gastric epithelial cells.
    Article Snippet: When appropriate, protein amount was estimated using Pierce BCA protein assay kit (Thermo Fisher Scientific).

    Article Title: Piezo1 ion channels are capable of conformational signaling
    Article Snippet: The membrane fraction was isolated from Neuro2A cells using the ProteoExtract® Subcellular Proteome Extraction Kit (Sigma) and concentrated to 1-2 mg/mL using a Pierce TM concentrator (3K molecular weight cutoff; ThermoFisher Scientific).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: ProteoExtract® Subcellular Proteome Extraction Kit , Millipore-Sigma , 539790.

    Article Title: Pre-clinical characterization of novel multi-client inhibitors of Sec61 with broad anti-tumor activity
    Article Snippet: 243 Proteomic profiling 244 Cell lines and PBMCs were treated for 24 hours with either KZR-834 or KZR-261 245 (250 nM) followed by subcellular fractionation of cytosolic (F1) and 246 membrane/organelle (F2) proteomes using the ProteoExtract® Subcellular 247 Proteome Extraction Kit (Calbiochem).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: Cytoplasm and membrane fractions were obtained using the ProteoExtract® Subcellular Proteome Extraction Kit (Millipore-Sigma] following manufacturer protocol.

    Article Title: Linoleic acid improves PIEZO2 dysfunction in a mouse model of Angelman Syndrome.
    Article Snippet: Transfected, Latrunculin A (1μM; 20–24 h)-treated, or LAsupplemented MCC13 cells were treated with ProteoExtract® Subcellular Proteome Extraction Kit (Millipore Sigma) to obtain cytosolic, membrane, and cytoskeletal fractions, according to the manufacturer’s instructions and elsewhere100.

    Cell Fractionation:

    Article Title: Two opposing gene expression patterns within ATRX aberrant neuroblastoma
    Article Snippet: Cell fractionation experiments were performed with the ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem®).

    Article Title: Helicobacter pylori regulates TIFA turnover in gastric epithelial cells.
    Article Snippet: When appropriate, protein amount was estimated using Pierce BCA protein assay kit (Thermo Fisher Scientific).

    Article Title: Piezo1 ion channels are capable of conformational signaling
    Article Snippet: The membrane fraction was isolated from Neuro2A cells using the ProteoExtract® Subcellular Proteome Extraction Kit (Sigma) and concentrated to 1-2 mg/mL using a Pierce TM concentrator (3K molecular weight cutoff; ThermoFisher Scientific).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: ProteoExtract® Subcellular Proteome Extraction Kit , Millipore-Sigma , 539790.

    Article Title: Pre-clinical characterization of novel multi-client inhibitors of Sec61 with broad anti-tumor activity
    Article Snippet: 243 Proteomic profiling 244 Cell lines and PBMCs were treated for 24 hours with either KZR-834 or KZR-261 245 (250 nM) followed by subcellular fractionation of cytosolic (F1) and 246 membrane/organelle (F2) proteomes using the ProteoExtract® Subcellular 247 Proteome Extraction Kit (Calbiochem).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: Cytoplasm and membrane fractions were obtained using the ProteoExtract® Subcellular Proteome Extraction Kit (Millipore-Sigma] following manufacturer protocol.

    Article Title: Linoleic acid improves PIEZO2 dysfunction in a mouse model of Angelman Syndrome.
    Article Snippet: Transfected, Latrunculin A (1μM; 20–24 h)-treated, or LAsupplemented MCC13 cells were treated with ProteoExtract® Subcellular Proteome Extraction Kit (Millipore Sigma) to obtain cytosolic, membrane, and cytoskeletal fractions, according to the manufacturer’s instructions and elsewhere100.

    Immunoprecipitation:

    Article Title: Two opposing gene expression patterns within ATRX aberrant neuroblastoma
    Article Snippet: Cell fractionation experiments were performed with the ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem®).

    Article Title: Helicobacter pylori regulates TIFA turnover in gastric epithelial cells.
    Article Snippet: When appropriate, protein amount was estimated using Pierce BCA protein assay kit (Thermo Fisher Scientific).

    Article Title: Piezo1 ion channels are capable of conformational signaling
    Article Snippet: The membrane fraction was isolated from Neuro2A cells using the ProteoExtract® Subcellular Proteome Extraction Kit (Sigma) and concentrated to 1-2 mg/mL using a Pierce TM concentrator (3K molecular weight cutoff; ThermoFisher Scientific).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: ProteoExtract® Subcellular Proteome Extraction Kit , Millipore-Sigma , 539790.

    Article Title: Pre-clinical characterization of novel multi-client inhibitors of Sec61 with broad anti-tumor activity
    Article Snippet: 243 Proteomic profiling 244 Cell lines and PBMCs were treated for 24 hours with either KZR-834 or KZR-261 245 (250 nM) followed by subcellular fractionation of cytosolic (F1) and 246 membrane/organelle (F2) proteomes using the ProteoExtract® Subcellular 247 Proteome Extraction Kit (Calbiochem).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: Cytoplasm and membrane fractions were obtained using the ProteoExtract® Subcellular Proteome Extraction Kit (Millipore-Sigma] following manufacturer protocol.

    Article Title: Linoleic acid improves PIEZO2 dysfunction in a mouse model of Angelman Syndrome.
    Article Snippet: Transfected, Latrunculin A (1μM; 20–24 h)-treated, or LAsupplemented MCC13 cells were treated with ProteoExtract® Subcellular Proteome Extraction Kit (Millipore Sigma) to obtain cytosolic, membrane, and cytoskeletal fractions, according to the manufacturer’s instructions and elsewhere100.

    Lysis:

    Article Title: Two opposing gene expression patterns within ATRX aberrant neuroblastoma
    Article Snippet: Cell fractionation experiments were performed with the ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem®).

    Article Title: Helicobacter pylori regulates TIFA turnover in gastric epithelial cells.
    Article Snippet: When appropriate, protein amount was estimated using Pierce BCA protein assay kit (Thermo Fisher Scientific).

    Article Title: Piezo1 ion channels are capable of conformational signaling
    Article Snippet: The membrane fraction was isolated from Neuro2A cells using the ProteoExtract® Subcellular Proteome Extraction Kit (Sigma) and concentrated to 1-2 mg/mL using a Pierce TM concentrator (3K molecular weight cutoff; ThermoFisher Scientific).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: ProteoExtract® Subcellular Proteome Extraction Kit , Millipore-Sigma , 539790.

    Article Title: Pre-clinical characterization of novel multi-client inhibitors of Sec61 with broad anti-tumor activity
    Article Snippet: 243 Proteomic profiling 244 Cell lines and PBMCs were treated for 24 hours with either KZR-834 or KZR-261 245 (250 nM) followed by subcellular fractionation of cytosolic (F1) and 246 membrane/organelle (F2) proteomes using the ProteoExtract® Subcellular 247 Proteome Extraction Kit (Calbiochem).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: Cytoplasm and membrane fractions were obtained using the ProteoExtract® Subcellular Proteome Extraction Kit (Millipore-Sigma] following manufacturer protocol.

    Article Title: Linoleic acid improves PIEZO2 dysfunction in a mouse model of Angelman Syndrome.
    Article Snippet: Transfected, Latrunculin A (1μM; 20–24 h)-treated, or LAsupplemented MCC13 cells were treated with ProteoExtract® Subcellular Proteome Extraction Kit (Millipore Sigma) to obtain cytosolic, membrane, and cytoskeletal fractions, according to the manufacturer’s instructions and elsewhere100.

    Centrifugation:

    Article Title: Two opposing gene expression patterns within ATRX aberrant neuroblastoma
    Article Snippet: Cell fractionation experiments were performed with the ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem®).

    Article Title: Helicobacter pylori regulates TIFA turnover in gastric epithelial cells.
    Article Snippet: When appropriate, protein amount was estimated using Pierce BCA protein assay kit (Thermo Fisher Scientific).

    Article Title: Piezo1 ion channels are capable of conformational signaling
    Article Snippet: The membrane fraction was isolated from Neuro2A cells using the ProteoExtract® Subcellular Proteome Extraction Kit (Sigma) and concentrated to 1-2 mg/mL using a Pierce TM concentrator (3K molecular weight cutoff; ThermoFisher Scientific).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: ProteoExtract® Subcellular Proteome Extraction Kit , Millipore-Sigma , 539790.

    Article Title: Pre-clinical characterization of novel multi-client inhibitors of Sec61 with broad anti-tumor activity
    Article Snippet: 243 Proteomic profiling 244 Cell lines and PBMCs were treated for 24 hours with either KZR-834 or KZR-261 245 (250 nM) followed by subcellular fractionation of cytosolic (F1) and 246 membrane/organelle (F2) proteomes using the ProteoExtract® Subcellular 247 Proteome Extraction Kit (Calbiochem).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: Cytoplasm and membrane fractions were obtained using the ProteoExtract® Subcellular Proteome Extraction Kit (Millipore-Sigma] following manufacturer protocol.

    Article Title: Linoleic acid improves PIEZO2 dysfunction in a mouse model of Angelman Syndrome.
    Article Snippet: Transfected, Latrunculin A (1μM; 20–24 h)-treated, or LAsupplemented MCC13 cells were treated with ProteoExtract® Subcellular Proteome Extraction Kit (Millipore Sigma) to obtain cytosolic, membrane, and cytoskeletal fractions, according to the manufacturer’s instructions and elsewhere100.

    Control:

    Article Title: Two opposing gene expression patterns within ATRX aberrant neuroblastoma
    Article Snippet: Cell fractionation experiments were performed with the ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem®).

    Article Title: Helicobacter pylori regulates TIFA turnover in gastric epithelial cells.
    Article Snippet: When appropriate, protein amount was estimated using Pierce BCA protein assay kit (Thermo Fisher Scientific).

    Article Title: Piezo1 ion channels are capable of conformational signaling
    Article Snippet: The membrane fraction was isolated from Neuro2A cells using the ProteoExtract® Subcellular Proteome Extraction Kit (Sigma) and concentrated to 1-2 mg/mL using a Pierce TM concentrator (3K molecular weight cutoff; ThermoFisher Scientific).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: ProteoExtract® Subcellular Proteome Extraction Kit , Millipore-Sigma , 539790.

    Article Title: Pre-clinical characterization of novel multi-client inhibitors of Sec61 with broad anti-tumor activity
    Article Snippet: 243 Proteomic profiling 244 Cell lines and PBMCs were treated for 24 hours with either KZR-834 or KZR-261 245 (250 nM) followed by subcellular fractionation of cytosolic (F1) and 246 membrane/organelle (F2) proteomes using the ProteoExtract® Subcellular 247 Proteome Extraction Kit (Calbiochem).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: Cytoplasm and membrane fractions were obtained using the ProteoExtract® Subcellular Proteome Extraction Kit (Millipore-Sigma] following manufacturer protocol.

    Article Title: Linoleic acid improves PIEZO2 dysfunction in a mouse model of Angelman Syndrome.
    Article Snippet: Transfected, Latrunculin A (1μM; 20–24 h)-treated, or LAsupplemented MCC13 cells were treated with ProteoExtract® Subcellular Proteome Extraction Kit (Millipore Sigma) to obtain cytosolic, membrane, and cytoskeletal fractions, according to the manufacturer’s instructions and elsewhere100.

    Generated:

    Article Title: Two opposing gene expression patterns within ATRX aberrant neuroblastoma
    Article Snippet: Cell fractionation experiments were performed with the ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem®).

    Article Title: Helicobacter pylori regulates TIFA turnover in gastric epithelial cells.
    Article Snippet: When appropriate, protein amount was estimated using Pierce BCA protein assay kit (Thermo Fisher Scientific).

    Article Title: Piezo1 ion channels are capable of conformational signaling
    Article Snippet: The membrane fraction was isolated from Neuro2A cells using the ProteoExtract® Subcellular Proteome Extraction Kit (Sigma) and concentrated to 1-2 mg/mL using a Pierce TM concentrator (3K molecular weight cutoff; ThermoFisher Scientific).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: ProteoExtract® Subcellular Proteome Extraction Kit , Millipore-Sigma , 539790.

    Article Title: Pre-clinical characterization of novel multi-client inhibitors of Sec61 with broad anti-tumor activity
    Article Snippet: 243 Proteomic profiling 244 Cell lines and PBMCs were treated for 24 hours with either KZR-834 or KZR-261 245 (250 nM) followed by subcellular fractionation of cytosolic (F1) and 246 membrane/organelle (F2) proteomes using the ProteoExtract® Subcellular 247 Proteome Extraction Kit (Calbiochem).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: Cytoplasm and membrane fractions were obtained using the ProteoExtract® Subcellular Proteome Extraction Kit (Millipore-Sigma] following manufacturer protocol.

    Article Title: Linoleic acid improves PIEZO2 dysfunction in a mouse model of Angelman Syndrome.
    Article Snippet: Transfected, Latrunculin A (1μM; 20–24 h)-treated, or LAsupplemented MCC13 cells were treated with ProteoExtract® Subcellular Proteome Extraction Kit (Millipore Sigma) to obtain cytosolic, membrane, and cytoskeletal fractions, according to the manufacturer’s instructions and elsewhere100.

    Recombinant:

    Article Title: Two opposing gene expression patterns within ATRX aberrant neuroblastoma
    Article Snippet: Cell fractionation experiments were performed with the ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem®).

    Article Title: Helicobacter pylori regulates TIFA turnover in gastric epithelial cells.
    Article Snippet: When appropriate, protein amount was estimated using Pierce BCA protein assay kit (Thermo Fisher Scientific).

    Article Title: Piezo1 ion channels are capable of conformational signaling
    Article Snippet: The membrane fraction was isolated from Neuro2A cells using the ProteoExtract® Subcellular Proteome Extraction Kit (Sigma) and concentrated to 1-2 mg/mL using a Pierce TM concentrator (3K molecular weight cutoff; ThermoFisher Scientific).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: ProteoExtract® Subcellular Proteome Extraction Kit , Millipore-Sigma , 539790.

    Article Title: Pre-clinical characterization of novel multi-client inhibitors of Sec61 with broad anti-tumor activity
    Article Snippet: 243 Proteomic profiling 244 Cell lines and PBMCs were treated for 24 hours with either KZR-834 or KZR-261 245 (250 nM) followed by subcellular fractionation of cytosolic (F1) and 246 membrane/organelle (F2) proteomes using the ProteoExtract® Subcellular 247 Proteome Extraction Kit (Calbiochem).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: Cytoplasm and membrane fractions were obtained using the ProteoExtract® Subcellular Proteome Extraction Kit (Millipore-Sigma] following manufacturer protocol.

    Article Title: Linoleic acid improves PIEZO2 dysfunction in a mouse model of Angelman Syndrome.
    Article Snippet: Transfected, Latrunculin A (1μM; 20–24 h)-treated, or LAsupplemented MCC13 cells were treated with ProteoExtract® Subcellular Proteome Extraction Kit (Millipore Sigma) to obtain cytosolic, membrane, and cytoskeletal fractions, according to the manufacturer’s instructions and elsewhere100.

    Cell Culture:

    Article Title: Two opposing gene expression patterns within ATRX aberrant neuroblastoma
    Article Snippet: Cell fractionation experiments were performed with the ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem®).

    Article Title: Helicobacter pylori regulates TIFA turnover in gastric epithelial cells.
    Article Snippet: When appropriate, protein amount was estimated using Pierce BCA protein assay kit (Thermo Fisher Scientific).

    Article Title: Piezo1 ion channels are capable of conformational signaling
    Article Snippet: The membrane fraction was isolated from Neuro2A cells using the ProteoExtract® Subcellular Proteome Extraction Kit (Sigma) and concentrated to 1-2 mg/mL using a Pierce TM concentrator (3K molecular weight cutoff; ThermoFisher Scientific).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: ProteoExtract® Subcellular Proteome Extraction Kit , Millipore-Sigma , 539790.

    Article Title: Pre-clinical characterization of novel multi-client inhibitors of Sec61 with broad anti-tumor activity
    Article Snippet: 243 Proteomic profiling 244 Cell lines and PBMCs were treated for 24 hours with either KZR-834 or KZR-261 245 (250 nM) followed by subcellular fractionation of cytosolic (F1) and 246 membrane/organelle (F2) proteomes using the ProteoExtract® Subcellular 247 Proteome Extraction Kit (Calbiochem).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: Cytoplasm and membrane fractions were obtained using the ProteoExtract® Subcellular Proteome Extraction Kit (Millipore-Sigma] following manufacturer protocol.

    Article Title: Linoleic acid improves PIEZO2 dysfunction in a mouse model of Angelman Syndrome.
    Article Snippet: Transfected, Latrunculin A (1μM; 20–24 h)-treated, or LAsupplemented MCC13 cells were treated with ProteoExtract® Subcellular Proteome Extraction Kit (Millipore Sigma) to obtain cytosolic, membrane, and cytoskeletal fractions, according to the manufacturer’s instructions and elsewhere100.

    Plasmid Preparation:

    Article Title: Two opposing gene expression patterns within ATRX aberrant neuroblastoma
    Article Snippet: Cell fractionation experiments were performed with the ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem®).

    Article Title: Helicobacter pylori regulates TIFA turnover in gastric epithelial cells.
    Article Snippet: When appropriate, protein amount was estimated using Pierce BCA protein assay kit (Thermo Fisher Scientific).

    Article Title: Piezo1 ion channels are capable of conformational signaling
    Article Snippet: The membrane fraction was isolated from Neuro2A cells using the ProteoExtract® Subcellular Proteome Extraction Kit (Sigma) and concentrated to 1-2 mg/mL using a Pierce TM concentrator (3K molecular weight cutoff; ThermoFisher Scientific).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: ProteoExtract® Subcellular Proteome Extraction Kit , Millipore-Sigma , 539790.

    Article Title: Pre-clinical characterization of novel multi-client inhibitors of Sec61 with broad anti-tumor activity
    Article Snippet: 243 Proteomic profiling 244 Cell lines and PBMCs were treated for 24 hours with either KZR-834 or KZR-261 245 (250 nM) followed by subcellular fractionation of cytosolic (F1) and 246 membrane/organelle (F2) proteomes using the ProteoExtract® Subcellular 247 Proteome Extraction Kit (Calbiochem).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: Cytoplasm and membrane fractions were obtained using the ProteoExtract® Subcellular Proteome Extraction Kit (Millipore-Sigma] following manufacturer protocol.

    Article Title: Linoleic acid improves PIEZO2 dysfunction in a mouse model of Angelman Syndrome.
    Article Snippet: Transfected, Latrunculin A (1μM; 20–24 h)-treated, or LAsupplemented MCC13 cells were treated with ProteoExtract® Subcellular Proteome Extraction Kit (Millipore Sigma) to obtain cytosolic, membrane, and cytoskeletal fractions, according to the manufacturer’s instructions and elsewhere100.

    Imaging:

    Article Title: Two opposing gene expression patterns within ATRX aberrant neuroblastoma
    Article Snippet: Cell fractionation experiments were performed with the ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem®).

    Article Title: Helicobacter pylori regulates TIFA turnover in gastric epithelial cells.
    Article Snippet: When appropriate, protein amount was estimated using Pierce BCA protein assay kit (Thermo Fisher Scientific).

    Article Title: Piezo1 ion channels are capable of conformational signaling
    Article Snippet: The membrane fraction was isolated from Neuro2A cells using the ProteoExtract® Subcellular Proteome Extraction Kit (Sigma) and concentrated to 1-2 mg/mL using a Pierce TM concentrator (3K molecular weight cutoff; ThermoFisher Scientific).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: ProteoExtract® Subcellular Proteome Extraction Kit , Millipore-Sigma , 539790.

    Article Title: Pre-clinical characterization of novel multi-client inhibitors of Sec61 with broad anti-tumor activity
    Article Snippet: 243 Proteomic profiling 244 Cell lines and PBMCs were treated for 24 hours with either KZR-834 or KZR-261 245 (250 nM) followed by subcellular fractionation of cytosolic (F1) and 246 membrane/organelle (F2) proteomes using the ProteoExtract® Subcellular 247 Proteome Extraction Kit (Calbiochem).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: Cytoplasm and membrane fractions were obtained using the ProteoExtract® Subcellular Proteome Extraction Kit (Millipore-Sigma] following manufacturer protocol.

    Article Title: Linoleic acid improves PIEZO2 dysfunction in a mouse model of Angelman Syndrome.
    Article Snippet: Transfected, Latrunculin A (1μM; 20–24 h)-treated, or LAsupplemented MCC13 cells were treated with ProteoExtract® Subcellular Proteome Extraction Kit (Millipore Sigma) to obtain cytosolic, membrane, and cytoskeletal fractions, according to the manufacturer’s instructions and elsewhere100.

    Blocking Assay:

    Article Title: Two opposing gene expression patterns within ATRX aberrant neuroblastoma
    Article Snippet: Cell fractionation experiments were performed with the ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem®).

    Article Title: Helicobacter pylori regulates TIFA turnover in gastric epithelial cells.
    Article Snippet: When appropriate, protein amount was estimated using Pierce BCA protein assay kit (Thermo Fisher Scientific).

    Article Title: Piezo1 ion channels are capable of conformational signaling
    Article Snippet: The membrane fraction was isolated from Neuro2A cells using the ProteoExtract® Subcellular Proteome Extraction Kit (Sigma) and concentrated to 1-2 mg/mL using a Pierce TM concentrator (3K molecular weight cutoff; ThermoFisher Scientific).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: ProteoExtract® Subcellular Proteome Extraction Kit , Millipore-Sigma , 539790.

    Article Title: Pre-clinical characterization of novel multi-client inhibitors of Sec61 with broad anti-tumor activity
    Article Snippet: 243 Proteomic profiling 244 Cell lines and PBMCs were treated for 24 hours with either KZR-834 or KZR-261 245 (250 nM) followed by subcellular fractionation of cytosolic (F1) and 246 membrane/organelle (F2) proteomes using the ProteoExtract® Subcellular 247 Proteome Extraction Kit (Calbiochem).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: Cytoplasm and membrane fractions were obtained using the ProteoExtract® Subcellular Proteome Extraction Kit (Millipore-Sigma] following manufacturer protocol.

    Article Title: Linoleic acid improves PIEZO2 dysfunction in a mouse model of Angelman Syndrome.
    Article Snippet: Transfected, Latrunculin A (1μM; 20–24 h)-treated, or LAsupplemented MCC13 cells were treated with ProteoExtract® Subcellular Proteome Extraction Kit (Millipore Sigma) to obtain cytosolic, membrane, and cytoskeletal fractions, according to the manufacturer’s instructions and elsewhere100.

    Gentle:

    Article Title: Two opposing gene expression patterns within ATRX aberrant neuroblastoma
    Article Snippet: Cell fractionation experiments were performed with the ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem®).

    Article Title: Helicobacter pylori regulates TIFA turnover in gastric epithelial cells.
    Article Snippet: When appropriate, protein amount was estimated using Pierce BCA protein assay kit (Thermo Fisher Scientific).

    Article Title: Piezo1 ion channels are capable of conformational signaling
    Article Snippet: The membrane fraction was isolated from Neuro2A cells using the ProteoExtract® Subcellular Proteome Extraction Kit (Sigma) and concentrated to 1-2 mg/mL using a Pierce TM concentrator (3K molecular weight cutoff; ThermoFisher Scientific).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: ProteoExtract® Subcellular Proteome Extraction Kit , Millipore-Sigma , 539790.

    Article Title: Pre-clinical characterization of novel multi-client inhibitors of Sec61 with broad anti-tumor activity
    Article Snippet: 243 Proteomic profiling 244 Cell lines and PBMCs were treated for 24 hours with either KZR-834 or KZR-261 245 (250 nM) followed by subcellular fractionation of cytosolic (F1) and 246 membrane/organelle (F2) proteomes using the ProteoExtract® Subcellular 247 Proteome Extraction Kit (Calbiochem).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: Cytoplasm and membrane fractions were obtained using the ProteoExtract® Subcellular Proteome Extraction Kit (Millipore-Sigma] following manufacturer protocol.

    Article Title: Linoleic acid improves PIEZO2 dysfunction in a mouse model of Angelman Syndrome.
    Article Snippet: Transfected, Latrunculin A (1μM; 20–24 h)-treated, or LAsupplemented MCC13 cells were treated with ProteoExtract® Subcellular Proteome Extraction Kit (Millipore Sigma) to obtain cytosolic, membrane, and cytoskeletal fractions, according to the manufacturer’s instructions and elsewhere100.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Two opposing gene expression patterns within ATRX aberrant neuroblastoma
    Article Snippet: Cell fractionation experiments were performed with the ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem®).

    Article Title: Helicobacter pylori regulates TIFA turnover in gastric epithelial cells.
    Article Snippet: When appropriate, protein amount was estimated using Pierce BCA protein assay kit (Thermo Fisher Scientific).

    Article Title: Piezo1 ion channels are capable of conformational signaling
    Article Snippet: The membrane fraction was isolated from Neuro2A cells using the ProteoExtract® Subcellular Proteome Extraction Kit (Sigma) and concentrated to 1-2 mg/mL using a Pierce TM concentrator (3K molecular weight cutoff; ThermoFisher Scientific).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: ProteoExtract® Subcellular Proteome Extraction Kit , Millipore-Sigma , 539790.

    Article Title: Pre-clinical characterization of novel multi-client inhibitors of Sec61 with broad anti-tumor activity
    Article Snippet: 243 Proteomic profiling 244 Cell lines and PBMCs were treated for 24 hours with either KZR-834 or KZR-261 245 (250 nM) followed by subcellular fractionation of cytosolic (F1) and 246 membrane/organelle (F2) proteomes using the ProteoExtract® Subcellular 247 Proteome Extraction Kit (Calbiochem).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: Cytoplasm and membrane fractions were obtained using the ProteoExtract® Subcellular Proteome Extraction Kit (Millipore-Sigma] following manufacturer protocol.

    Article Title: Linoleic acid improves PIEZO2 dysfunction in a mouse model of Angelman Syndrome.
    Article Snippet: Transfected, Latrunculin A (1μM; 20–24 h)-treated, or LAsupplemented MCC13 cells were treated with ProteoExtract® Subcellular Proteome Extraction Kit (Millipore Sigma) to obtain cytosolic, membrane, and cytoskeletal fractions, according to the manufacturer’s instructions and elsewhere100.

    In Situ:

    Article Title: Two opposing gene expression patterns within ATRX aberrant neuroblastoma
    Article Snippet: Cell fractionation experiments were performed with the ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem®).

    Article Title: Helicobacter pylori regulates TIFA turnover in gastric epithelial cells.
    Article Snippet: When appropriate, protein amount was estimated using Pierce BCA protein assay kit (Thermo Fisher Scientific).

    Article Title: Piezo1 ion channels are capable of conformational signaling
    Article Snippet: The membrane fraction was isolated from Neuro2A cells using the ProteoExtract® Subcellular Proteome Extraction Kit (Sigma) and concentrated to 1-2 mg/mL using a Pierce TM concentrator (3K molecular weight cutoff; ThermoFisher Scientific).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: ProteoExtract® Subcellular Proteome Extraction Kit , Millipore-Sigma , 539790.

    Article Title: Pre-clinical characterization of novel multi-client inhibitors of Sec61 with broad anti-tumor activity
    Article Snippet: 243 Proteomic profiling 244 Cell lines and PBMCs were treated for 24 hours with either KZR-834 or KZR-261 245 (250 nM) followed by subcellular fractionation of cytosolic (F1) and 246 membrane/organelle (F2) proteomes using the ProteoExtract® Subcellular 247 Proteome Extraction Kit (Calbiochem).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: Cytoplasm and membrane fractions were obtained using the ProteoExtract® Subcellular Proteome Extraction Kit (Millipore-Sigma] following manufacturer protocol.

    Article Title: Linoleic acid improves PIEZO2 dysfunction in a mouse model of Angelman Syndrome.
    Article Snippet: Transfected, Latrunculin A (1μM; 20–24 h)-treated, or LAsupplemented MCC13 cells were treated with ProteoExtract® Subcellular Proteome Extraction Kit (Millipore Sigma) to obtain cytosolic, membrane, and cytoskeletal fractions, according to the manufacturer’s instructions and elsewhere100.

    Staining:

    Article Title: Two opposing gene expression patterns within ATRX aberrant neuroblastoma
    Article Snippet: Cell fractionation experiments were performed with the ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem®).

    Article Title: Helicobacter pylori regulates TIFA turnover in gastric epithelial cells.
    Article Snippet: When appropriate, protein amount was estimated using Pierce BCA protein assay kit (Thermo Fisher Scientific).

    Article Title: Piezo1 ion channels are capable of conformational signaling
    Article Snippet: The membrane fraction was isolated from Neuro2A cells using the ProteoExtract® Subcellular Proteome Extraction Kit (Sigma) and concentrated to 1-2 mg/mL using a Pierce TM concentrator (3K molecular weight cutoff; ThermoFisher Scientific).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: ProteoExtract® Subcellular Proteome Extraction Kit , Millipore-Sigma , 539790.

    Article Title: Pre-clinical characterization of novel multi-client inhibitors of Sec61 with broad anti-tumor activity
    Article Snippet: 243 Proteomic profiling 244 Cell lines and PBMCs were treated for 24 hours with either KZR-834 or KZR-261 245 (250 nM) followed by subcellular fractionation of cytosolic (F1) and 246 membrane/organelle (F2) proteomes using the ProteoExtract® Subcellular 247 Proteome Extraction Kit (Calbiochem).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: Cytoplasm and membrane fractions were obtained using the ProteoExtract® Subcellular Proteome Extraction Kit (Millipore-Sigma] following manufacturer protocol.

    Article Title: Linoleic acid improves PIEZO2 dysfunction in a mouse model of Angelman Syndrome.
    Article Snippet: Transfected, Latrunculin A (1μM; 20–24 h)-treated, or LAsupplemented MCC13 cells were treated with ProteoExtract® Subcellular Proteome Extraction Kit (Millipore Sigma) to obtain cytosolic, membrane, and cytoskeletal fractions, according to the manufacturer’s instructions and elsewhere100.

    Software:

    Article Title: Two opposing gene expression patterns within ATRX aberrant neuroblastoma
    Article Snippet: Cell fractionation experiments were performed with the ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem®).

    Article Title: Helicobacter pylori regulates TIFA turnover in gastric epithelial cells.
    Article Snippet: When appropriate, protein amount was estimated using Pierce BCA protein assay kit (Thermo Fisher Scientific).

    Article Title: Piezo1 ion channels are capable of conformational signaling
    Article Snippet: The membrane fraction was isolated from Neuro2A cells using the ProteoExtract® Subcellular Proteome Extraction Kit (Sigma) and concentrated to 1-2 mg/mL using a Pierce TM concentrator (3K molecular weight cutoff; ThermoFisher Scientific).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: ProteoExtract® Subcellular Proteome Extraction Kit , Millipore-Sigma , 539790.

    Article Title: Pre-clinical characterization of novel multi-client inhibitors of Sec61 with broad anti-tumor activity
    Article Snippet: 243 Proteomic profiling 244 Cell lines and PBMCs were treated for 24 hours with either KZR-834 or KZR-261 245 (250 nM) followed by subcellular fractionation of cytosolic (F1) and 246 membrane/organelle (F2) proteomes using the ProteoExtract® Subcellular 247 Proteome Extraction Kit (Calbiochem).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: Cytoplasm and membrane fractions were obtained using the ProteoExtract® Subcellular Proteome Extraction Kit (Millipore-Sigma] following manufacturer protocol.

    Article Title: Linoleic acid improves PIEZO2 dysfunction in a mouse model of Angelman Syndrome.
    Article Snippet: Transfected, Latrunculin A (1μM; 20–24 h)-treated, or LAsupplemented MCC13 cells were treated with ProteoExtract® Subcellular Proteome Extraction Kit (Millipore Sigma) to obtain cytosolic, membrane, and cytoskeletal fractions, according to the manufacturer’s instructions and elsewhere100.

    High Content Screening:

    Article Title: Two opposing gene expression patterns within ATRX aberrant neuroblastoma
    Article Snippet: Cell fractionation experiments were performed with the ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem®).

    Article Title: Helicobacter pylori regulates TIFA turnover in gastric epithelial cells.
    Article Snippet: When appropriate, protein amount was estimated using Pierce BCA protein assay kit (Thermo Fisher Scientific).

    Article Title: Piezo1 ion channels are capable of conformational signaling
    Article Snippet: The membrane fraction was isolated from Neuro2A cells using the ProteoExtract® Subcellular Proteome Extraction Kit (Sigma) and concentrated to 1-2 mg/mL using a Pierce TM concentrator (3K molecular weight cutoff; ThermoFisher Scientific).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: ProteoExtract® Subcellular Proteome Extraction Kit , Millipore-Sigma , 539790.

    Article Title: Pre-clinical characterization of novel multi-client inhibitors of Sec61 with broad anti-tumor activity
    Article Snippet: 243 Proteomic profiling 244 Cell lines and PBMCs were treated for 24 hours with either KZR-834 or KZR-261 245 (250 nM) followed by subcellular fractionation of cytosolic (F1) and 246 membrane/organelle (F2) proteomes using the ProteoExtract® Subcellular 247 Proteome Extraction Kit (Calbiochem).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: Cytoplasm and membrane fractions were obtained using the ProteoExtract® Subcellular Proteome Extraction Kit (Millipore-Sigma] following manufacturer protocol.

    Article Title: Linoleic acid improves PIEZO2 dysfunction in a mouse model of Angelman Syndrome.
    Article Snippet: Transfected, Latrunculin A (1μM; 20–24 h)-treated, or LAsupplemented MCC13 cells were treated with ProteoExtract® Subcellular Proteome Extraction Kit (Millipore Sigma) to obtain cytosolic, membrane, and cytoskeletal fractions, according to the manufacturer’s instructions and elsewhere100.

    Transfection:

    Article Title: Two opposing gene expression patterns within ATRX aberrant neuroblastoma
    Article Snippet: Cell fractionation experiments were performed with the ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem®).

    Article Title: Helicobacter pylori regulates TIFA turnover in gastric epithelial cells.
    Article Snippet: When appropriate, protein amount was estimated using Pierce BCA protein assay kit (Thermo Fisher Scientific).

    Article Title: Piezo1 ion channels are capable of conformational signaling
    Article Snippet: The membrane fraction was isolated from Neuro2A cells using the ProteoExtract® Subcellular Proteome Extraction Kit (Sigma) and concentrated to 1-2 mg/mL using a Pierce TM concentrator (3K molecular weight cutoff; ThermoFisher Scientific).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: ProteoExtract® Subcellular Proteome Extraction Kit , Millipore-Sigma , 539790.

    Article Title: Pre-clinical characterization of novel multi-client inhibitors of Sec61 with broad anti-tumor activity
    Article Snippet: 243 Proteomic profiling 244 Cell lines and PBMCs were treated for 24 hours with either KZR-834 or KZR-261 245 (250 nM) followed by subcellular fractionation of cytosolic (F1) and 246 membrane/organelle (F2) proteomes using the ProteoExtract® Subcellular 247 Proteome Extraction Kit (Calbiochem).

    Article Title: Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis
    Article Snippet: Cytoplasm and membrane fractions were obtained using the ProteoExtract® Subcellular Proteome Extraction Kit (Millipore-Sigma] following manufacturer protocol.

    Article Title: Linoleic acid improves PIEZO2 dysfunction in a mouse model of Angelman Syndrome.
    Article Snippet: Transfected, Latrunculin A (1μM; 20–24 h)-treated, or LAsupplemented MCC13 cells were treated with ProteoExtract® Subcellular Proteome Extraction Kit (Millipore Sigma) to obtain cytosolic, membrane, and cytoskeletal fractions, according to the manufacturer’s instructions and elsewhere100.



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